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使用两步PCR和下一代16S rRNA基因测序进行微生物群分析
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使用两步PCR和下一代16S rRNA基因测序进行微生物群分析

Article DOI: 10.3791/59980-v • 11:22 min • October 15th, 2019
October 15th, 2019 •
1, 2, 1, 1,2,3,4
1Department of Pathology, University of Iowa, 2Medical Scientist Training Program, University of Iowa, 3Graduate Program in Immunology, University of Iowa, 4Graduate Program in Molecular Medicine, University of Iowa

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这里描述的是使用16S rRNA基因组测序和利用免费工具进行分析的微生物群分析的简化标准操作程序。该协议将帮助那些对微生物群领域较新的研究人员，以及那些需要更新方法以更高分辨率实现细菌分析的研究人员。

Tags

生物学， 问题 152， 粪便 DNA 提取， 库准备， 16S 可变区域， 16S rRNA 下一代测序， 肠道微生物群
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