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Biochemistry

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JoVE Journal Biochemistry
Kvantitativ analyse af cellulære lipidom af Saccharomyces Cerevisiae Ved hjælp af flydende kromatografi kombineret med tandem massespektrometri
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Kvantitativ analyse af cellulære lipidom af Saccharomyces Cerevisiae Ved hjælp af flydende kromatografi kombineret med tandem massespektrometri

Article DOI: 10.3791/60616-v • 08:56 min • March 8th, 2020
March 8th, 2020 •
1, 2, 1, 1
1Department of Biology, Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry, Concordia University

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Vi præsenterer en protokol ved hjælp af flydende kromatografi kombineret med tandem massespektrometri at identificere og kvantificere større cellulære lipider i Saccharomyces cerevisiae. Den beskrevne metode til en kvantitativ vurdering af større lipidklasser i en gærcelle er alsidig, robust og følsom.

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Biokemi lipider cellulær lipidom unesterified (gratis) fedtsyrer glycerophospholipider neutrale lipider sphingolipider ceramider kardiolipiner lipid ekstraktion lipidomics flydende kromatografi massespektrometri
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