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Journal / Genetics / Screening CRISPR/Cas9 in vivo per valutare…
JoVE Journal
Genetics

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JoVE Journal Genetics
Screening CRISPR/Cas9 in vivo per valutare contemporaneamente la funzione genica nella pelle del topo e nella cavità orale
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Screening CRISPR/Cas9 in vivo per valutare contemporaneamente la funzione genica nella pelle del topo e nella cavità orale

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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Qui descriviamo una metodologia di screening CRISPR/Cas9 in vivo rapida e diretta utilizzando iniezioni lentivirali embrionali guidate da ultrasuoni in utero per valutare contemporaneamente le funzioni di diversi geni nella pelle e nella cavità orale dei topi immunocompetenti.

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Questo mese a JoVE Numero 165 CRISPR in vivo micro iniezione guidata da ultrasuoni coda lunga libreria CRISPR modelli di topo di cancro schermo rapido in vivo
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