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CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
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JoVE Journal Genetics
CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum

CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum

DOI:
10.3791/57747-v

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09:25 min

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September 18, 2018

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, , , ,
1Department of Cellular Biology,University of Georgia, 2Center for Tropical and Emerging Global Diseases,University of Georgia

Chapters

  • 00:04Title
  • 00:30Choosing a gRNA Sequence
  • 01:31Precipitating DNA for Transfection
  • 03:00Transfecting RBCs with the CRISPR/Cas9 Plasmids
  • 06:49Cloning Parasites by Limiting Dilution
  • 08:16Results: Tagging and Conditional Knockdown of PfHsp70x
  • 09:01Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for generating glmS-based conditional knockdown mutants in Plasmodium falciparum using CRISPR/Cas9 genome editing.

Tags

Keywords: CRISPR/Cas9Gene EditingConditional MutantsPlasmodium FalciparumMalaria ParasiteProtein FunctionKnockdownGRNATransfectionRBCsCytomix BufferRPMI

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