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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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JoVE Journal Genetics
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

DOI:
10.3791/61693-v

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07:52 min

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November 02, 2020

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, , , ,
1Centre for Molecular and Systems Biology,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics,University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University

Chapters

  • 00:05Introduction
  • 00:40Ultra-sound Guided Surgery and Injection
  • 05:44Results: In vivo CRISPR Screen for Tumor Suppressor Genes
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent mice.

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Keywords: CRISPR/Cas9In Vivo ScreeningMouse SkinOral CavityTumor Suppressor GenesHead And Neck CancerGene FunctionSkin ExpressionTargeted CRISPR LibraryPregnancyEmbryonic Day 9.5UltrasoundAnesthesiaIncisionPeritoneumUterine HornSilicone MembranePBSModeling Clay

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