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Fotobleken Analyses (FRAP & FLIP) om chromatine eiwit Dynamics Meet in Living Embryonale stamcellen

Article DOI: 10.3791/2696-v • 09:18 min • June 29th, 2011
June 29th, 2011 •
1, 1
1Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem

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We beschrijven fotobleken methoden, met inbegrip Fluorescence Recovery After Photobleaching (FRAP) en fluorescentie Loss In Photobleaching (FLIP) om chromatine eiwit dynamiek monitor in embryonale stamcellen (ES) cellen. Chromatine eiwit dynamiek, die wordt beschouwd als een van de middelen om chromatine plasticiteit bestuderen, wordt versterkt in de pluripotente cellen.

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Developmental Biology Live imaging FRAP FLIP embryonale stamcellen (ES)-cellen chromatine chromatine plasticiteit eiwit dynamica
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