Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Creating Adhesive and Soluble Gradients for…
JoVE Journal
Bioengineering

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Bioengineering
Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
Journal (Bioengineering)
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
Journal (Bioengineering)
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
 

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Article DOI: 10.3791/50310-v • 13:10 min • April 4th, 2013
April 4th, 2013 •
1, 1, 2, 1, 1, 2, 1, 1
1Centre for Vascular Research and Australian Centre for Nanomedicine, The University of New South Wales, 2School of Chemistry and Australian Centre for Nanomedicine, The University of New South Wales

챕터

요약
Automatic Translation

A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.

Tags

Adhesive Gradients Soluble Gradients Cell Migration Fluorescence Microscopy Microfluidic Chamber Live Cell Imaging Copolymer Poly-L-lysine Polyethylene Glycol Passivation Non-specific Adsorption Biomolecules Cells Microcontact Printing Dip Pen Lithography Streptavidin Biotinylated Peptide Arginine-glycine-aspartic Acid (RGD) Microfluidic Device Chemoattractant Fetal Bovine Serum (FBS)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter