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Biochemistry

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JoVE Journal Biochemistry
Ein Tailored HPLC Reinigungsprotokoll, dass die Renditen hochreine Amyloid Beta 42 und Amyloid Beta 40 Peptides, Fähig zur Oligomerbildung
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Ein Tailored HPLC Reinigungsprotokoll, dass die Renditen hochreine Amyloid Beta 42 und Amyloid Beta 40 Peptides, Fähig zur Oligomerbildung

Article DOI: 10.3791/55482-v • 06:34 min • March 27th, 2017
March 27th, 2017 •
1, 1, 1, 1
1Department of Chemistry, University of California, Santa Cruz

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Wir berichten hier über eine HPLC-Reinigung Protokoll zugeschnitten, die hochreine Beta-Amyloid-42 (Aß42) und Beta-Amyloid-40 (Aβ40) Peptide, der fähig Oligomerbildung ergibt. Amyloid beta ist ein hoch Aggregation neigen, hydrophoben Peptid in Alzheimer-Krankheit in Verbindung gebracht. Die amyloidogenen Natur des Peptids macht seine Reinigung eine Herausforderung.

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Biochemie Heft 121 Amyloid beta Peptidreinigung High Performance Liquid Chromatography Alzheimer-Krankheit Oligomerbildung PICUP
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