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Biochemistry

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JoVE Journal Biochemistry
Kalibrering-fri In Vitro kvantificering af Protein Homo-oligomerisering ved hjælp af kommercielle instrumentering og gratis, Open Source lysstyrke analyse Software
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Kalibrering-fri In Vitro kvantificering af Protein Homo-oligomerisering ved hjælp af kommercielle instrumentering og gratis, Open Source lysstyrke analyse Software

Article DOI: 10.3791/58157-v • 08:22 min • July 17th, 2018
July 17th, 2018 •
1, 1, 2, 2, 2, 1,2,3,4
1Cellular Imaging Group, Wellcome Centre Human Genetics, University of Oxford, 2Division of Structural Biology, Wellcome Centre Human Genetics, University of Oxford, 3Dynamic Structural Virology Group, Biocruces Health Research Centre, 4IKERBASQUE, Basque Foundation for Science

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Denne protokol beskriver en kalibrering-fri tilgang til kvantificering af protein homo-oligomerisering in vitro- baseret på fluorescens udsving spektroskopi ved hjælp af kommercielle lys scanning mikroskopi. Den korrekte anskaffelse indstillinger og analysemetoder, der er vist.

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Biokemi sag 137 Dimerization kalibrering gratis spektroskopi fluorescens udsving antallet og lysstyrke protein sammenlægning enkelt molekyle mikroskopi
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