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Immunology and Infection

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Het meten van Erythroocyte Complement Receptor 1 met behulp van Flow Cytometrie

Article DOI: 10.3791/60810-v • 07:20 min • May 19th, 2020
May 19th, 2020 •
1,2, 3, 2,4, 2,4, 2, 5,6
1Oncogeriatric Coordination Unit, Reims University Hospitals, Maison Blanche Hospital, 2Faculty of Medicine, University of Reims Champagne-Ardenne, 3URCACyt, Flow cytometry technical platform, University of Reims Champagne-Ardenne, 4Department of Immunology, Reims University Hospitals, Robert Debre Hospital, 5Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 6Faculty of Medicine, University of Reims Champagne-Ardenne

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Het doel van deze methode is om de CR1-dichtheid in de erytrocyten van elk onderwerp te bepalen door te vergelijken met drie proefpersonen waarvan de erytrocyten CR1-dichtheid bekend is. De methode maakt gebruik van flow cytometrie na immunostaining van de erytrocyten van de proefpersonen door een anti-CR1 monoklonale antilichaam gekoppeld aan een versterkt systeem met behulp van fycoerythrin (PE).

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Immunologie en infectie nummer 159 CR1 CD35 aanvulling C3b/C4b receptor CR1 dichtheid polymorfisme flow cytometrie de ziekte van Alzheimer Systemische lupus erythematosus malaria
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