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Isolement de cellules endothéliales de la lumière des artères carotides de souris pour des expériences multi-omiques unicellulaires
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Isolement de cellules endothéliales de la lumière des artères carotides de souris pour des expériences multi-omiques unicellulaires

Article DOI: 10.3791/63128-v • 09:12 min • October 4th, 2021
October 4th, 2021 •
*1, *1, 1, 1, 1, 1,2
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2Division of Cardiology, Georgia Institute of Technology and Emory University
* These authors contributed equally

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Nous présentons une méthode pour isoler les cellules endothéliales et les noyaux de la lumière des artères carotides de souris exposées à des conditions d’écoulement stables ou perturbées pour effectuer des expériences omiques unicellulaires.

Tags

Biologie numéro 176 Cellules artérielles Cellules endothéliales RNAseq unicellulaire ATACseq unicellulaire Athérosclérose
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