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Biochemistry

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JoVE Journal Biochemistry
体外 使用分析、下拉和伴侣测定表征组蛋白伴侣
Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
Journal (Biochemistry)
Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
Journal (Biochemistry)
"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
 
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体外 使用分析、下拉和伴侣测定表征组蛋白伴侣

Article DOI: 10.3791/63218-v • 08:16 min • December 29th, 2021
December 29th, 2021 •
*1,2, *1,2, 1,3, 1,2, 1,2, 1, 1,4, 1,5, 1, 1
1Institute of Life Sciences, 2Regional Centre for Biotechnology, 3School of Biotechnology, KIIT University, 4Department of Molecular Biophysics and Biochemistry, Yale University, 5Department of Pharmacology, University of Vermont College of Medicine
* These authors contributed equally

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该协议描述了一系列方法，包括分析体积排阻色谱以研究组蛋白伴侣寡聚化和稳定性，下拉测定以揭示组蛋白伴侣 - 组蛋白相互作用，AUC用于分析蛋白质复合物的化学计量，以及组蛋白伴侣测定以在 体外功能表征假定的组蛋白伴侣。

Tags

生物化学，第178期，组蛋白伴侣，核小体组装，染色质，核蓝蛋白，NAP，质粒超螺旋，分析超速离心
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