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Journal / Biochemistry / Purificação dos canais potenciais do receptor…
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Biochemistry

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JoVE Journal Biochemistry
Purificação dos canais potenciais do receptor transitório Endogenous Drosophila
Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling
Journal (Biochemistry)
Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Journal (Biochemistry)
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
 
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Purificação dos canais potenciais do receptor transitório Endogenous Drosophila

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Com base no mecanismo de montagem do complexo proteico INAD, neste protocolo, foi desenvolvida uma purificação de afinidade modificada mais estratégia de concorrência para purificar o canal endogenso Drosophila TRP.

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Bioquímica Edição 178 purificação de proteínas Drosophila canal TRP INAD
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