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Biochemistry

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JoVE Journal Biochemistry
Purificación de los canales potenciales del receptor transitorio de Drosophila endógeno
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Journal (Biochemistry)
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
 
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Purificación de los canales potenciales del receptor transitorio de Drosophila endógeno

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Basado en el mecanismo de ensamblaje del complejo de proteínas INAD, en este protocolo, se desarrolló una estrategia modificada de purificación de afinidad más competencia para purificar el canal endógeno Drosophila TRP.

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Bioquímica Número 178 purificación de proteínas Drosophila canal TRP INAD
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