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Immunology and Infection

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Kultivierung von Lymphozyten in simulierter Schwerelosigkeit mit einem rotierenden Zellkultursystem
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Kultivierung von Lymphozyten in simulierter Schwerelosigkeit mit einem rotierenden Zellkultursystem

Article DOI: 10.3791/63296-v • 09:28 min • August 25th, 2022
August 25th, 2022 •
1,2, 1,2,4, 1,3
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre

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Dies ist eine Schritt-für-Schritt-Anleitung für die Verwendung eines kommerziell erhältlichen rotierenden Zellkultursystems zur Kultivierung von Lymphozyten in simulierter Schwerelosigkeit mit speziellen Einweg-Kulturgefäßen. Diese Kultivierungsmethode kann auf jede Suspensionszellkultur angewendet werden.

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Retraktion Ausgabe 186 Suspensionszellkultur Lymphozyten simulierte Schwerelosigkeit zeitgemittelte Schwerkraftvektor-Nullifikation 2D-Klinostat rotierendes Zellkultursystem rotierende Wandgefäßvorrichtung
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