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Journal / Neuroscience / In situ Hybridisering kombinerad med…
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Neuroscience

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In situ Hybridisering kombinerad med immunhistokemi i kryosektionerade zebrafiskembryon
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In situ Hybridisering kombinerad med immunhistokemi i kryosektionerade zebrafiskembryon

Article DOI: 10.3791/63715-v • 07:36 min • March 3rd, 2022
March 3rd, 2022 •
*1,2, *3, 2, 4, 2, 1, 2,3, 5, 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University
* These authors contributed equally

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Detta protokoll beskriver hur man får bilder genom att kombinera in situ hybridisering och immunhistokemi av zebrafisk embryonala sektioner. In situ-hybridisering utfördes före kryosektion, följt av antikroppsfärgning. Det är användbart att detektera uttrycksmönstren för två gener i zebrafisk om det finns en brist på antikroppar.

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Denna månad i JoVE nummer 181 In situ-hybridisering immunhistokemi zebrafisk mRNA antikropp protein
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