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Application Fluorescence Resonance Energy Transfer (FRET) pour examiner effecteur translocation efficacité par
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Application Fluorescence Resonance Energy Transfer (FRET) pour examiner effecteur translocation efficacité par

Article DOI: 10.3791/54210-v • 10:29 min • July 6th, 2016
July 6th, 2016 •
1, 1, 1
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne

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L'étude des interactions entre les bactéries pathogènes et leurs hôtes est un domaine important de la recherche biologique. Ici, nous décrivons les techniques nécessaires pour mesurer la translocation effecteur par Coxiella burnetii lors de l' inactivation de gène siRNA en utilisant un substrat BlaM.

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Infection numéro 113 les interactions hôte-pathogène les systèmes de sécrétion bactériens essai de translocation β-lactamase substrat BlaM fluorescence microbiologie bactéries pathogènes intracellulaires, Silençage génique siRNA biologie moléculaire
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