Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Desmontaje genético directo de las células madre…
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
Desmontaje genético directo de las células madre neurales de la médula espinal de Axolotl a través de la electroporación de los complejos de proteína-gRNA CAS9
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Journal (Genetics)
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Journal (Genetics)
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
 
Click here for the English version

Desmontaje genético directo de las células madre neurales de la médula espinal de Axolotl a través de la electroporación de los complejos de proteína-gRNA CAS9

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Aquí se presenta un protocolo para realizar el desomo genético con tiempo y espacio restringido en las médulas espinales de axolotl inyectando el complejo CAS9-gRNA en el canal central de la médula espinal seguido de electroporación.

Tags

Genética Número 149 axolotl médula espinal regeneración CRISPR-Cas9 células madre neurales nocaut genético
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter