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第4章
配体结合位点
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their…
蛋白质-蛋白质相互作用界面
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most…
保守结合位点
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein…
平衡结合常数和结合强度
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is…
辅助因子和辅酶
Enzymes require additional components for proper function. There are two such classes of molecules: cofactors and coenzymes. Cofactors are metallic…
变构规则
Allosteric regulation of enzymes occurs when the binding of a molecule to a different location from the active site causes a change in enzymatic…
配体结合与连接
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the…
Allosteric Proteins-ATCase
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism…
合作变构过渡
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand…
磷酸化
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These…
蛋白激酶和磷酸酶
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation,…
GTPases蛋白家族及其监管
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as…
共价连接的蛋白质调节剂
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an…
具有可互换部分的蛋白质复合物
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s…
机械蛋白质功能
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical…
结构蛋白功能
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major…
蛋白质网络
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other…
用放射性标记的ATP测定蛋白激酶活性
Protein kinases are able to govern large-scale cellular changes in response to complex arrays of stimuli, and much effort has been directed at…
综合结构质谱分析蛋白质结构和蛋白质配体配合物
Proteins are an important class of biological macromolecules that play many key roles in cellular functions including gene expression, catalyzing…
使用多路复用共免疫沉淀对蛋白质相互作用网络动力学进行量化
Dynamic protein-protein interactions control cellular behavior, from motility to DNA replication to signal transduction. However, monitoring dynamic…

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