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使用多路复用共免疫沉淀对蛋白质相互作用网络动力学进行量化
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
DOI:

00:07 min

August 21, 2019

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Chapters

  • 00:04Title
  • 00:59Quantitative Multiplex Immunoprecipitation (Day 1)
  • 02:19Running the Assay (Day 2)
  • 04:40Data Analysis
  • 06:10Positive Hits and Visualization
  • 06:57Results: Representative Protein Interaction Network Dynamic Quantification
  • 07:20Conclusion

Summary

Automatic Translation

定量多路复用免疫沉淀 (QMI) 使用流式细胞测定技术,灵敏地检测两个样本之间靶向蛋白-蛋白质相互作用的丰度差异。QMI可以使用少量的生物材料进行,不需要基因工程标签,并可以适应任何先前定义的蛋白质相互作用网络。

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