Thin Layer Chromatography for Mixture Separation

Thin layer chromatography separates mixtures of non-volatile compounds. It uses a TLC plate with a thin layer of adsorbent material as the stationary phase. The adsorbent layer is fixed to a solid support such as plastic, aluminum, or glass.

The sample and a reference compound are first dissolved...

Thin layer chromatography, or TLC, is a chromatographic method used to separate mixtures of non-volatile compounds, commonly used in organic chemistry.

TLC is performed on a glass- or plastic-backed plate. A baseline is marked on the plate, along with labels. The mixture being examined and reference compounds are dissolved in an appropriat...