Materials
| Name | Company | Catalog Number | Comments |
| Yeast | Fisher Scientific | S80245-3 | Active Dry |
| Fly Food | Home made | Corn meal, sugar | Standard |
| Honey | Dutch Gold | From the store | Clover |
| Vials | Fisher Scientific | AS514 | |
| Cotton plug/Foam stopper | Fisher Scientific | 14-127-40A | |
| Spatula | Fisher Scientific | 21-401-15 | |
| Pyrex dissecting dish | Fisher Scientific | 50-930-382 | 9-well |
| Microscope slides pre-cleaned | Fisher Scientific | 7101 | 25.4 mm x 76.2 mm |
| Glass coverslips | Pearl | D12544 | 22 x 50 |
| Glass coverslips | Pearl | G12544 | 22 x 60 |
| Pasteur Pipette | J H Berge | 71-5200-05 | |
| 100 mm Tweezers | Sigma-Aldrich | T-4662 | Style # 5 |
| Wash Bottle | Fisher Scientific | 03-409-22A | Fisherbrand |
| Kim Wipess | Fisher Scientific | 34155 | Kimberly Clark |
| Paper Towel | Fisher Scientific | Fisher X-101-C | 1/8 x 13 1/8 in. |
| Leica stereomicroscope | Empire Imaging Systems, INC. | 10446208 | MZFLIII |
| Zeiss Stereomicroscope | Carl Zeiss, Inc. | 000000-1006-126 | "Stemi" 1000 or 2000-C |
| Light Source -LED Gooseneck illuminator | Fisher Scientific | 12563501 | |
| Stage | Carl Zeiss, Inc. | ||
| Zeiss Laser 510 Scanning Confocal Microscope | Carl Zeiss, Inc. | ||
| Incubator | Thermo Fisher Scientific, Inc. | 815 | |
| Dulbecco’s Phosphate-Buffered Salt Solution 1X | Fisher Scientific | MT-21-030-CM Mediatech | |
| Ethanol | Fisher Scientific | 64-17-5 | 99.5 % |
| Formaldehyde (37% w/w) | Fisher Scientific | F79-1 | |
| H–chst 33258 | Molecular Probes, Life Technologies | H-1398 | 0.2 μg/ml Excitation 352 nm; Emission 461 nm |
| Rhodamine phalloidin | Molecular Probes, Life Technologies | R415 | 200 units/ml (6.6 μM) Excitation 546 nm; Emission 565 nm |
| Alexa Fluor 488 phalloidin | Molecular Probes, Life Technologies | A22289 | Excitation 495 nm; Emission 518 nm 300 units/ml |
| CO2 tank | TW Smith | ||
| Anti-Spz antibody | Gift Dr. C. Hashimoto (Yale) | ||
| Secondary antibody TRITC-conjugated donkey anti-rabbit, concentration 1:50. | Jackson ImmunoResearch | 711-165-152 | Detected at Excitation 546 nm; Emission 565 nm |
| Antifade | MP Biomedicals | ICN10274790 | 0.08 mg of N-propyl gallate in 20 ml of 50% glycerol in 1X PBS |
| Wasp Strains | Fly Strains | ||
| L. victoriae16 | y w | ||
| L. boulardi 171 | UAS-GFP-Dorsal17 | ||
| L. heterotoma2 | SerpentHemoGal413 | ||
| L. heterotoma 141 | MSNF9-moCherry14 | ||
| Trichopria drosophilae | MSNF-GFP15 | ||
| G. xanthopoda18 | y w Serpent-Gal4 UAS GFP-Dorsal/Basc4 | ||
| y w ; Drosomycin-GFP/CyO y+12 | |||
References
- Schlenke, T. A., Morales, J., Govind, S., Clark, A. G. Contrasting infection strategies in generalist and specialist wasp parasitoids of Drosophila melanogaster. PLoS Pathog. 3, 1486-1501 (2007).
- Chiu, H., Morales, J., Govind, S. Identification and immuno-electron microscopy localization of p40, a protein component of immunosuppressive virus-like particles from Leptopilina heterotoma, a virulent parasitoid wasp of Drosophila. J. Gen. Virol. 87, 461-470 (2006).
- Gueguen, G., Rajwani, R., Paddibhatla, I., Morales, J., Govind, S. VLPs of Leptopilina boulardi share biogenesis and overall stellate morphology with VLPs of the heterotoma clade. Virus Res. 160, 159-165 (2011).
- Paddibhatla, I., Lee, M. J., Kalamarz, M. E., Ferrarese, R., Govind, S. Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae. PLoS Pathog. 6, e1001234 (2010).
- Sorrentino, R. P., Carton, Y., Govind, S. Cellular immune response to parasite infection in the Drosophila lymph gland is developmentally regulated. Dev. Biol. , 243-265 (2002).
- Sorrentino, R. P., Melk, J. P., Govind, S. Genetic analysis of contributions of dorsal group and JAK-Stat92E pathway genes to larval hemocyte concentration and the egg encapsulation response in Drosophila. Genetics. 166, 1343-1356 (2004).
- Jung, S. H., Evans, C. J., Uemura, C., Banerjee, U. The Drosophila lymph gland as a developmental model of hematopoiesis. Development. 132, 2521-2533 (2005).
- Krzemien, J., Crozatier, M., Vincent, A. Ontogeny of the Drosophila larval hematopoietic organ, hemocyte homeostasis and the dedicated cellular immune response to parasitism. Int. J. Dev. Biol. 54, 1117-1125 (2010).
- Martinez-Agosto, J. A., Mikkola, H. K., Hartenstein, V., Banerjee, U. The hematopoietic stem cell and its niche: a comparative view. Genes Dev. 21, 3044-3060 (2007).
- Lemaitre, B., Hoffmann, J. The host defense of Drosophila melanogaster. Annu. Rev. Immunol. 25, 697-743 (2007).
- Schlegel, A., Stainier, D. Y. Lessons from "lower" organisms: what worms, flies, and zebrafish can teach us about human energy metabolism. PLoS Genet. 3, e199 (2007).
- Ferrandon, D., Jung, A. C., Criqui, M., Lemaitre, B., Uttenweiler-Joseph, S., Michaut, L., Reichhart, J., Hoffmann, J. A. A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. EMBO J. 17, 1217-1227 (1998).
- Bruckner, K., Kockel, L., Duchek, P., Luque, C. M., Rorth, P., Perrimon, N. The PDGF/VEGF receptor controls blood cell survival in Drosophila. Dev. Cell. 7, 73-84 (2004).
- Tokusumi, T., Shoue, D. A., Tokusumi, Y., Stoller, J. R., Schulz, R. A. New hemocyte-specific enhancer-reporter transgenes for the analysis of hematopoiesis in Drosophila. Genesis. 47, 771-774 (2009).
- Tokusumi, T., Sorrentino, R. P., Russell, M., Ferrarese, R., Govind, S., Schulz, R. A. Characterization of a lamellocyte transcriptional enhancer located within the misshapen gene of Drosophila melanogaster. PLoS One. 4, e6429 (2009).
- Morales, J., Chiu, H., Oo, T., Plaza, R., Hoskins, S., Govind, S. Biogenesis, structure, and immune-suppressive effects of virus-like particles of a Drosophila parasitoid, Leptopilina victoriae. J. Insect Physiol. 51, 181-195 (2005).
- Bettencourt, R., Asha, H., Dearolf, C., Ip, Y. T. Hemolymph-dependent and -independent responses in Drosophila immune tissue. J. Cell Biochem. 92, 849-863 (2004).
- Melk, J. P., Govind, S. Developmental analysis of Ganaspis xanthopoda, a larval parasitoid of Drosophila melanogaster. J. Exp. Biol. 202, 1885-1896 (1999).
