Насекомые<em> Galleria mellonella</em> Как мощная модель инфекции по расследованию бактериального патогенеза
Summary
Устные и внутри haemocolic заражения личинок восковой моли больше<em> Galleria mellonella</em> Описано. Это насекомое может быть использована для изучения факторов вирулентности энтомопатогенных, а также млекопитающих патогенных бактерий. Разведение насекомых, способы заражения и примеры<em> В естественных условиях</em> Анализ описаны.
Abstract
The study of bacterial virulence often requires a suitable animal model. Mammalian models of infection are costly and may raise ethical issues. The use of insects as infection models provides a valuable alternative. Compared to other non-vertebrate model hosts such as nematodes, insects have a relatively advanced system of antimicrobial defenses and are thus more likely to produce information relevant to the mammalian infection process. Like mammals, insects possess a complex innate immune system1. Cells in the hemolymph are capable of phagocytosing or encapsulating microbial invaders, and humoral responses include the inducible production of lysozyme and small antibacterial peptides2,3. In addition, analogies are found between the epithelial cells of insect larval midguts and intestinal cells of mammalian digestive systems. Finally, several basic components essential for the bacterial infection process such as cell adhesion, resistance to antimicrobial peptides, tissue degradation and adaptation to oxidative stress are likely to be important in both insects and mammals1. Thus, insects are polyvalent tools for the identification and characterization of microbial virulence factors involved in mammalian infections.
Larvae of the greater wax moth Galleria mellonella have been shown to provide a useful insight into the pathogenesis of a wide range of microbial infections including mammalian fungal (Fusarium oxysporum, Aspergillus fumigatus, Candida albicans) and bacterial pathogens, such as Staphylococcus aureus, Proteus vulgaris, Serratia marcescens Pseudomonas aeruginosa, Listeria monocytogenes or Enterococcus faecalis4-7. Regardless of the bacterial species, results obtained with Galleria larvae infected by direct injection through the cuticle consistently correlate with those of similar mammalian studies: bacterial strains that are attenuated in mammalian models demonstrate lower virulence in Galleria, and strains causing severe human infections are also highly virulent in the Galleria model8-11. Oral infection of Galleria is much less used and additional compounds, like specific toxins, are needed to reach mortality.
G. mellonella larvae present several technical advantages: they are relatively large (last instar larvae before pupation are about 2 cm long and weight 250 mg), thus enabling the injection of defined doses of bacteria; they can be reared at various temperatures (20 °C to 30 °C) and infection studies can be conducted between 15 °C to above 37 °C12,13, allowing experiments that mimic a mammalian environment. In addition, insect rearing is easy and relatively cheap. Infection of the larvae allows monitoring bacterial virulence by several means, including calculation of LD5014, measurement of bacterial survival15,16 and examination of the infection process17. Here, we describe the rearing of the insects, covering all life stages of G. mellonella. We provide a detailed protocol of infection by two routes of inoculation: oral and intra haemocoelic. The bacterial model used in this protocol is Bacillus cereus, a Gram positive pathogen implicated in gastrointestinal as well as in other severe local or systemic opportunistic infections18,19.
Protocol
Representative Results
Discussion
Использование насекомых и особенно в личиночной стадии, так как инфекция моделей для нескольких патогенов, становятся частыми. Модель выбора для некоторых аспектов Drosophila (Fly Model), используемая как взрослых, так и личиночные 1,2 сцене. Чешуекрылых насекомых G. mellonella также бы…
Disclosures
The authors have nothing to disclose.
Acknowledgements
Мы хотели бы поблагодарить Элизабет Guillemet, Кристоф Buisson и Людовик Bridoux за отличную техническую помощь. Мы в большом долгу перед Сильви Salamitou и Синда Fedhila для начальной настройки системы.
Materials
| Name of the reagent | Company | Catalogue number | Comments (optional) |
| Wax and pollen | La Ruche Roanaise | 303000 | Any honey producer |
| Automated syringe pump | KD Scientific | KDS 100 | |
| Syringe 1 ml | Terumo | BS 01T | |
| Needle 0.45 x 12 mm | Terumo | NN 2613R | |
| Petri dish 5 cm | VWR | 89000-300 | |
| Needle 30G, 25 mm hypodermic | Burkard Mfg. Co. Ltd. | PDE0005 |
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