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Neuroscience

الليزر مسح ضوئي للدوائر الدماغ الأمامي المستهدفة Optogenetically

Published: December 27, 2013 doi: 10.3791/50915
Automatic Translation
1, 2, 1, 2
1Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 2Department of Neurobiology, University of Chicago

Materials

Name Company Catalog Number Comments
Polybrene Santa Cruz Biotech SC134220
Paraformaldehyde 32% solution EMS 15714
VECTASHIELD Vector Labs H-1400
AAV viral constructs UNC Vector Core NA
Mouse stereotactic headholder Stoelting 51730
Nanocool injector Harvard Apparatus 703040
UV laser: 355 nm, Nd:YVO4; 1 W; DPSS Lasers, Inc 3500
Galvonometers Cambridge Scanning 6210
mirrors Cambridge Scanning 6m2003S-A
mount Cambridge Scanning 6102103L
cable Cambridge Scanning 6010-19-120
driver/controller Cambridge Scanning 67121-1
Shutter - ZM coated (UV-compatible) Uniblitz L23-ZM2
Shutter Controller Uniblitz VMM-D1
Current Amplifier Stanford Research Systems SR570
6713 board National Instruments 777741-01
2110 break-out box National Instruments 777643-01
PCI-MIO-16E-4 National Instruments 777383-01
BNC-2090 National Instruments 777270-01
Cables National Instruments 184749-01
MATLAB Mathworks
TCS SP2 Leica Microsystems

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References

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  16. Petreanu, L., Huber, D., Sobczyk, A., Svoboda, K. Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Na. Neurosci. 10, 663-668 (2007).
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الليزر مسح ضوئي للدوائر الدماغ الأمامي المستهدفة Optogenetically
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Cite this Article

Lee, C. C., Lam, Y. W., Imaizumi,… More

Lee, C. C., Lam, Y. W., Imaizumi, K., Sherman, S. M. Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits. J. Vis. Exp. (82), e50915, doi:10.3791/50915 (2013).

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