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الليزر مسح ضوئي للدوائر الدماغ الأمامي المستهدفة Optogenetically

Published: December 27, 2013 doi: 10.3791/50915


Name Company Catalog Number Comments
Polybrene Santa Cruz Biotech SC134220
Paraformaldehyde 32% solution EMS 15714
VECTASHIELD Vector Labs H-1400
AAV viral constructs UNC Vector Core NA
Mouse stereotactic headholder Stoelting 51730
Nanocool injector Harvard Apparatus 703040
UV laser: 355 nm, Nd:YVO4; 1 W; DPSS Lasers, Inc 3500
Galvonometers Cambridge Scanning 6210
mirrors Cambridge Scanning 6m2003S-A
mount Cambridge Scanning 6102103L
cable Cambridge Scanning 6010-19-120
driver/controller Cambridge Scanning 67121-1
Shutter - ZM coated (UV-compatible) Uniblitz L23-ZM2
Shutter Controller Uniblitz VMM-D1
Current Amplifier Stanford Research Systems SR570
6713 board National Instruments 777741-01
2110 break-out box National Instruments 777643-01
PCI-MIO-16E-4 National Instruments 777383-01
BNC-2090 National Instruments 777270-01
Cables National Instruments 184749-01
MATLAB Mathworks
TCS SP2 Leica Microsystems



  1. Tye, K. M., Deisseroth, K. Optogenetic investigation of neural circuits underlying brain disease in animal models. Nat. Rev. Neurosci. 13, 251-266 (2012).
  2. Bernstein, J. G., Boyden, E. S. Optogenetic tools for analyzing the neural circuits of behavior. Trends Cog. Sci. 15, 592-600 (2011).
  3. Yizhar, O., Fenno, L. E., Davidson, T. J., Mogri, M., Deisseroth, K. Optogenetics in neural systems. Neuron. 71, 9-34 (2011).
  4. Nagel, G., et al. Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc. Natl. Acad. Sci. U.S.A. 100, 13940-13945 (2003).
  5. Zemelman, B. V., Lee, G. A., Ng, M., Miesenbock, G. Selective photostimulation of genetically ChARGed neurons. Neuron. 33, 15-22 (2001).
  6. Gradinaru, V., Thompson, K. R., Deisseroth, K. eNpHR: a Natronomonas halorhodopsin enhanced for optogenetic applications. Brain Cell Biol. 36, 129-139 (2008).
  7. Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., Deisseroth, K. M. illisecond-timescale genetically targeted optical control of neural activity. Nat. Neurosci. 8, 1263-1268 (2005).
  8. Lanyi, J. K. Halorhodopsin: A light-driven chloride ion pump. Ann. Rev. Biophys. Biophys. Chem. 15, 11-28 (1986).
  9. Han, X., Boyden, E. S. Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. PLoS One. 2, (2007).
  10. Zhao, S., et al. Cell type-specific channelrhodpsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nat. Methods. 8, 745-755 (2011).
  11. Madisen, L., et al. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nat. Neurosci. 15, 793-802 (2012).
  12. Fenno, L., Yizhar, O., Deisseroth, K. The development and application of optogenetics. Annu. Rev. Neurosci. 34, 389-412 (2011).
  13. Gradinaru, V., Mogri, M., Thompson, K. R., Henderson, J. M., Deisseroth, K. Optical deconstruction of parkinsonian neural circuit. Science. 324, 354-359 (2009).
  14. Olsen, S. R., Bortone, D. S., Adesnik, H., Scanziani, M. Gain control by layer six in cortical circuits of vision. Nature. 483, 47-52 (2012).
  15. Zhang, F., et al. Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures. Nat. Protoc. 5, 439-456 (2010).
  16. Petreanu, L., Huber, D., Sobczyk, A., Svoboda, K. Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Na. Neurosci. 10, 663-668 (2007).
  17. Cruikshank, S. J., Urabe, H., Nurmikko, A. V., Connors, B. W. Pathway-specific feedforward circuits between thalamus and neocortex revealed by selective optical stimulation of axons. Neuron. 65, 230-245 (2010).
  18. Lee, C. C., Lam, Y. W., Sherman, S. M. Intracortical convergence of layer 6 neurons. Neuroreport. 23, 736-740 (2012).
  19. Mancuso, J. J., et al. Optogenetic probing of functional brain circuitry. Exp. Physiol. 96, 26-33 (2011).
  20. Valley, M., Wagner, S., Gallarda, B. W., Lledo, P. Using affordable LED arrays for photo-stimulation of neurons. J. Vis. Exp. (57), (2011).
  21. Gong, S., et al. Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs. J. Neurosci. 27, 9817-9823 (2007).
  22. Cox, C. L., Sherman, S. M. Control of dendritic outputs of inhibitory interneurons in the lateral geniculate nucleus. Neuron. 27, 597-610 (2000).
  23. Lam, Y. W., Sherman, S. M. Mapping by laser photostimulation of connections between the thalamic reticular and ventral posterior lateral nuclei in the rat. J. Neurophysiol. 94, 2472-2483 (2005).
  24. Lam, Y. W., Sherman, S. M. Functional organization of the somatosensory cortical layer 6 feedback to the thalamus. Cereb Cortex. 20, 13-24 (2010).
  25. Shepherd, G. M., Pologruto, T. A., Svoboda, K. Circuit analysis of experience-dependent plasticity in the developing rat barrel cortex. Neuron. 38, 277-289 (2003).
  26. Suter, B. A., et al. Ephus: multipurpose data acquisition software for neuroscience experiments. Front Neural Circuits. 4, (2010).
  27. Lee, C. C., Sherman, S. M. Topography and physiology of ascending streams in the auditory tectothalamic pathway. Proc. Natl. Acad. Sci. U.S.A. 107, 372-377 (2010).
  28. Lee, C. C., Sherman, S. M. Intrinsic modulators of auditory thalamocortical transmission. Hear Res. , 287-2843 (2012).
  29. Shepherd, G. M. Circuit mapping by ultraviolet uncaging of glutamate. , Cold Spring. 998-1004 (2012).
  30. ScanImage, Ephus, and other DAQ software [Internet]. , Janelia Farms. Ashburn (VA). Available from: http://www.ephus.org (2003).
  31. UNC Gene Therapy Center [Internet]. , University of North Carolina. Chapel Hill (NC). Available from: http://genetherapy.unc.edu/services.htm (2003).
  32. Optogenetics Resource Center [Internet]. , Stanford University. Stanford (CA). Available from: http://www.stanford.edu/group/dlab/optogenetics (2003).
  33. Synthetic Neurobiology Group [Internet]. , MIT. Cambridge (MA). Available from: http://syntheticneurobiology.org (2003).
  34. Mutant Mouse Regional Resource Centers [Internet]. , NIH. Bethesda (MD). Available from: http://www.mmrrc.org (2003).
  35. GENSAT Brain Atlas [Internet]. , NIH. Bethesda (MD). Available from: http://www.gensat.org/cre.jsp (2003).
الليزر مسح ضوئي للدوائر الدماغ الأمامي المستهدفة Optogenetically
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Cite this Article

Lee, C. C., Lam, Y. W., Imaizumi,More

Lee, C. C., Lam, Y. W., Imaizumi, K., Sherman, S. M. Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits. J. Vis. Exp. (82), e50915, doi:10.3791/50915 (2013).

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