JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JoVE Journal > Biology
Protocol
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Biology
Monitoring Actin Disassembly with Time-lapse Microscopy
Published: November 08, 2006
doi:
10.3791/66
Hao Yuan Kueh
1
Dept. of Systems Biology
,
Harvard Medical School
Protocol
Construct a flow chamber as shown in the video. Make sure that the parafilm seal is tight and use washed coverslips.
Incubate actin-binding agent in the chamber for 5-10′.
Block non-specific binding sites on the glass coverslip with a blocking protein.
Polymerize actin inside the chamber by flowing in G-actin in polymerizing buffer.
Washout unpolymerized actin by flowing in excess buffer.
Tags
Monitoring
Actin Disassembly
Time-lapse Microscopy
Play Video
PDF
DOI
Cite This Article
Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy.
J. Vis. Exp.
(1), e66, doi:10.3791/66 (2006).
Copy Citation
Download Citation
Reprints and Permissions
View Video
✖
To prove you're not a robot, please enter the text in the image below
Email:
Enable Javascript for audio controls
Enter Captcha text here:
Submit