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JoVE Journal Biology
Souris d'isolement surrénales cellules chromaffines
 
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Souris d'isolement surrénales cellules chromaffines

Article doi: 10.3791/129
January 5th, 2007 •
1, 2,3, 4, 1, 2,3
1Department of Physiology and Biophysics, University of California, Irvine (UCI), 2Department of Physiology and Biophysics, University of Southern California, Keck School of Medicine, 3Zilkha Neurogenetic Institute, University of Southern California, Keck School of Medicine, 4Department of Developmental and Cell Biology, University of California, Irvine (UCI)

Summary
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January 5th, 2007

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Surrénales médullaires systèmes de culture de cellules chromaffines sont extrêmement utiles pour l'étude de l'excitation-sécrétion de couplage dans une dans la mise en in vitro. Ce protocole illustre la méthode utilisée pour disséquer les glandes surrénales et ensuite isoler la région médullaire en enlevant le cortex surrénalien. La digestion de la moelle dans les cellules chromaffines unique est alors démontrée.

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