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鼠标肾上腺嗜铬细胞分离
JoVE Journal
Biology
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JoVE Journal Biology
Mouse Adrenal Chromaffin Cell Isolation
DOI:

18:30 min

January 05, 2007

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Chapters

  • 00:45Tools and reagents
  • 01:09Dissection procedure
  • 05:04Isolation of medullae
  • 09:03Enzymatic digestion of tissue
  • 16:18Plating cells

Summary

Automatic Translation

肾上腺髓质嗜铬细胞培养系统,在体外设置为激发分泌耦合研究是非常有用的。该协议说明了用于解剖肾上腺然后隔离剥离肾上腺皮质髓质地区的方法。髓质消化成单个的嗜铬细胞,然后证明。

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