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鼠标肾上腺嗜铬细胞分离
JoVE Journal
Biology
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JoVE Journal
Biology
Mouse Adrenal Chromaffin Cell Isolation
Please note that all translations are automatically generated.
Click here for the English version.
鼠标肾上腺嗜铬细胞分离
DOI:
10.3791/129-v
•
18:30 min
•
January 05, 2007
•
Aaron Kolski-Andreaco
,
Haijiang Cai
3
,
D. Spencer Currle
,
K. George Chandy
,
Robert H. Chow
3
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
,
2
Department of Physiology and Biophysics
,
University of Southern California, Keck School of Medicine
,
3
Zilkha Neurogenetic Institute
,
University of Southern California, Keck School of Medicine
,
4
Department of Developmental and Cell Biology
,
University of California, Irvine (UCI)
Chapters
00:45
Tools and reagents
01:09
Dissection procedure
05:04
Isolation of medullae
09:03
Enzymatic digestion of tissue
16:18
Plating cells
Summary
Automatic Translation
English (Original)
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Automatic Translation
肾上腺髓质嗜铬细胞培养系统,在体外设置为激发分泌耦合研究是非常有用的。该协议说明了用于解剖肾上腺然后隔离剥离肾上腺皮质髓质地区的方法。髓质消化成单个的嗜铬细胞,然后证明。
Tags
Mouse
Adrenal
Chromaffin Cell
Isolation
Culture System
Excitation-secretion Coupling
In Vitro
Protocol
Dissect
Adrenals
Isolate
Medullary Region
Adrenal Cortex
Digestion
Single Chromaffin Cells
Article
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