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Biology

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Mouse Adrenal Chromaffin Cell Isolation
 
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Mouse Adrenal Chromaffin Cell Isolation

Article doi: 10.3791/129
January 5th, 2007

Summary January 5th, 2007

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Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.

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