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Chapters
- 00:00Title
- 00:37Introduction
- 01:18Isolation of Adult Cardiac Myocytes from Rat Heart by Langendorrf Preparation
- 08:02Primary Cell Culture and Adenoviral Transduction
- 09:45Confocal Imaging of 3D Subcellular Structures
- 11:09Imaging of Adult Rat Cardiac Myocytes Representative Results
- 11:43Conclusion
Adulto miócitos cardíacos são células primárias que podem ser isoladas de corações de animais e cultivados por vários dias. Dentro deste período a cultura de transferência de gene adenoviral podem ser usadas para expressar biossensores geneticamente codificado (GEBS) ou proteínas de fusão fluorescente. Ambas as abordagens permitem investigações celular por meio de microscopia confocal.
Tags
IsolationGenetic ManipulationAdult Cardiac MyocytesConfocal ImagingEmbryonic CellsNeonatal Muscle CellsWorking HeartCardiac Cellular PhysiologyPathophysiologyPharmaceutical InvestigationsTransgenic Animal ModelsLong Term CultureAdenoviral Gene TransferGenetically Encoded BiosensorsFluorescent Fusion ProteinsProtein OverexpressionProtein DownregulationRNAiSubcellular Structure StainingGolgi VisualizationConfocal ImagingZ-stacks ReconstructionCellular Cardiology
