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Chapters
- 00:00Title
- 00:37Introduction
- 01:18Isolation of Adult Cardiac Myocytes from Rat Heart by Langendorrf Preparation
- 08:02Primary Cell Culture and Adenoviral Transduction
- 09:45Confocal Imaging of 3D Subcellular Structures
- 11:09Imaging of Adult Rat Cardiac Myocytes Representative Results
- 11:43Conclusion
Adult Herzmuskelzellen sind primäre Zellen, die aus tierischen Herzen isoliert und kultiviert für mehrere Tage. Innerhalb dieses Zeitraums Kultur adenoviralen Gentransfer können verwendet werden, um genetisch kodierte Biosensoren (GEBS) oder fluoreszierende Fusionsproteine zu exprimieren. Beide Ansätze erlauben zellulären Untersuchungen mittels der konfokalen Mikroskopie.
Tags
IsolationGenetic ManipulationAdult Cardiac MyocytesConfocal ImagingEmbryonic CellsNeonatal Muscle CellsWorking HeartCardiac Cellular PhysiologyPathophysiologyPharmaceutical InvestigationsTransgenic Animal ModelsLong Term CultureAdenoviral Gene TransferGenetically Encoded BiosensorsFluorescent Fusion ProteinsProtein OverexpressionProtein DownregulationRNAiSubcellular Structure StainingGolgi VisualizationConfocal ImagingZ-stacks ReconstructionCellular Cardiology
