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Neuroscience

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JoVE Journal Neuroscience
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Bang-Sensitive Assay for the Study of Seizure-Like Behavior in Drosophila (Video) | JoVE

Monitoring Epileptiform Events in Drosophila via Calcium Imaging

Article DOI: 10.3791/200733-v • 01:18 min • October 13th, 2023
October 13th, 2023 •
1, 2, 2, 2, 3,4, 1
1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, 2The Second Clinical Medicine School of Guangzhou Medical University, 3Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University

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Ex vivo calciumbeeldvorming Drosophila-model Epilepsie Neurologische aandoening Terugkerende aanvallen Genetische oorsprong Beeldvormingstechnologie Genetisch gecodeerde calciumindicatoren GCaMP6 Hersenbrede resolutie Resolutie van één cel Moleculaire genetica Gedragstesten Protocol Volwassen Drosophila Epileptiforme activiteiten Confocale microscoop Neurale activiteit Bang-gevoelige aanvalsachtige gedragstest Cac-genknockdown-vliegen Abnormale calciumactiviteiten Pathogene genscreening
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