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Cancer Research

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JoVE Journal Cancer Research
Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation (Video) | JoVE
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Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation (Video) | JoVE

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

Article DOI: 10.3791/200792-v • 03:39 min • October 27th, 2023
October 27th, 2023 •
*1,2,3, *1, 4, 2, 1, 4,5,6, 1,2,3
1Department of Radiation Oncology, University of Kansas Cancer Center, 2Department of Cancer Biology, University of Kansas Cancer Center, 3BioEngineering Program, University of Kansas, 4Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine, University of Kansas Medical Center
* These authors contributed equally

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Rapide In Vitro Évaluation de la cytotoxicité Jurkat Récepteur antigénique chimérique Imagerie fluorescente Cellules CAR-T Domaine de ciblage Fragment variable à chaîne unique Linker intra-chaîne Domaine charnière Domaine transmembranaire Domaine costimulateur Construction CAR Destruction médiée par CAR Cellules CAR-T Processus chronophage Processus coûteux Constructions de test Investissement en temps et en matériel Plate-forme Constructions CAR optimisées pour les charnières Cellules Jurkat Absorption des lentivirus Transduction CAR Imageur fluorescent Cytolyse lymphocytes T dérivés de PBMC
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