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Journal
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Immunology and Infection
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质粒DNA重组流感病毒的一代
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Generation of Recombinant Influenza Virus from Plasmid DNA
Please note that all translations are automatically generated.
Click here for the English version.
质粒DNA重组流感病毒的一代
DOI:
10.3791/2057-v
•
11:31 min
•
August 03, 2010
•
Luis Martínez-Sobrido
,
Adolfo García-Sastre
1
Department of Microbiology and Immunology
,
University of Rochester School of Medicine and Dentistry
,
2
Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute
,
Mount Sinai School of Medicine
Chapters
00:04
Title
01:00
Introduction
01:29
Influenza Virus Rescue Transfection
04:54
Infecting MDCK Cells & TC Harvest
06:14
Egg Infection & Allonotic Fluid Harvest
07:55
HA Assay
08:55
Confirmation of Rescue
10:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
质粒DNA的A型流感病毒的救援是一个基本的和必要的实验技术,使流感的研究人员,以产生重组病毒在流感病毒的生物学特性研究的多个方面,被视为潜在的载体或疫苗使用。
Tags
Recombinant Influenza Virus
Plasmid DNA
Reverse Genetics
Influenza Research
Infectious Viruses
Viral Proteins
Mutations
Viral Replication
Pathogenesis
Viral Vectors
Foreign Proteins
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