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Neuroscience
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髄芽腫の幹細胞の単離、濃縮、およびメンテナンス
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
髄芽腫の幹細胞の単離、濃縮、およびメンテナンス
DOI:
10.3791/2086-v
•
06:32 min
•
September 01, 2010
•
Xi Huang
,
Tatiana Ketova
,
Ying LItingtung
,
Chin Chiang
1
Department of Cell and Developmental Biology
,
Vanderbilt University
Chapters
00:00
Title
00:59
Introduction
01:20
Micro-dissection of Tumor-bearing Cerebellum and Dissociation of Tumor Tissue
03:34
Enrichment, Maintenance, and Expansion of Medulloblastoma Tumor Cells by Serial Passages
04:26
Immunofluorescent Staining of Cultured Medulloblastoma Cells
04:53
Morphology, Lineage Differentiation, and Expression of Stem Cells Markers
06:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、分離、濃縮、および異所性ソニックヘッジホッグ経路の活性をもつ変異マウス由来の髄芽腫の腫瘍幹細胞の維持を説明します。
Tags
Isolation
Enrichment
Maintenance
Medulloblastoma Stem Cells
Brain Tumors
Neurosphere Assays
Neural Stem Cells
Tumorigenesis
Tumor Progression
Shh Signaling
Cerebellar Development
Cancer Stem Cells
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