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Isolering, anrikning och underhåll av celler medulloblastom Stem
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
DOI:

06:32 min

September 01, 2010

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Chapters

  • 00:00Title
  • 00:59Introduction
  • 01:20Micro-dissection of Tumor-bearing Cerebellum and Dissociation of Tumor Tissue
  • 03:34Enrichment, Maintenance, and Expansion of Medulloblastoma Tumor Cells by Serial Passages
  • 04:26Immunofluorescent Staining of Cultured Medulloblastoma Cells
  • 04:53Morphology, Lineage Differentiation, and Expression of Stem Cells Markers
  • 06:17Conclusion

Summary

Automatic Translation

Detta protokoll beskriver isolering, anrikning och underhåll av stamceller medulloblastom tumörceller från muterade möss med ektopisk Sonic Hedgehog pathway aktivitet.

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