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赤痢アメーバのDNAとtRNAメチルトランスフェラーゼDnmt2（Ehmeth）酵素を用いたin vitro tRNAのメチル化アッセイに

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Immunology and Infection

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JoVE Journal Immunology and Infection

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme

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赤痢アメーバのDNAとtRNAメチルトランスフェラーゼDnmt2（Ehmeth）酵素を用いたin vitro tRNAのメチル化アッセイに

DOI:

•

12:36 min

•

October 19, 2010

•

Ayala Tovy, Benjamin Hofmann, Mark Helm, Serge Ankri

¹Faculty of Medicine, Rappaport Institute,Technion – Israel Institute of Technology, ²The Pharmacy and Biochemistry Institute,Johannes Gutenberg University

Chapters

00:00Title
01:43tRNA Preparation
05:28Expression and Purification of the Recombinant E. histolytica Dnmt2 (Ehmeth) Protein in E. coli BL21
08:06 in vitro tRNA Methylation Assay
10:30Representative Results
12:02Conclusion

Summary

Automatic Translation

このプロトコルは、のための合成のtRNA基質の調製を記載し<em>赤痢アメーバ</em> DNA / tRNAのメチルトランスフェラーゼ2（Dnmt2）ホモログのEhmethとそのメチルトランスフェラーゼ活性の測定。この実験的なアプローチは、他のDnmt2タンパク質の活性を調べるために使用することができます。

Tags

In Vitro TRNA Methylation Assay Entamoeba Histolytica DNA Methyltransferase Dnmt2 Ehmeth Enzyme Protozoan Parasites Amebiasis Infectious Agents Clinical Manifestations 5-methylcytosine (5mC)Single DNA Methyltransferase Control Of Repetitive Elements Dual DNA/tRNAAsp Methyltransferase Activity Functional Significance TRNA Methyltransferase Activity Dnmt2 Proteins

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