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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
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In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme

Article DOI: 10.3791/2390-v • 12:36 min • October 19th, 2010
October 19th, 2010 •
1, 2, 2, 1
1Faculty of Medicine, Rappaport Institute, Technion - Israel Institute of Technology, 2The Pharmacy and Biochemistry Institute, Johannes Gutenberg University

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This protocol describes the preparation of a synthetic tRNA substrate for the Entamoeba histolytica DNA/tRNA methyltransferase 2 (Dnmt2) homolog Ehmeth and the measure of its methyltransferase activity. This experimental approach can be used for investigating the activity of other Dnmt2 proteins.

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In Vitro TRNA Methylation Assay Entamoeba Histolytica DNA Methyltransferase Dnmt2 Ehmeth Enzyme Protozoan Parasites Amebiasis Infectious Agents Clinical Manifestations 5-methylcytosine (5mC) Single DNA Methyltransferase Control Of Repetitive Elements Dual DNA/tRNAAsp Methyltransferase Activity Functional Significance TRNA Methyltransferase Activity Dnmt2 Proteins
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