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Entamoeba histolytica DNA와 tRNA Methyltransferase Dnmt2 (Ehmeth) 효소를 체외 tRNA의 Methylation 분석에서
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
DOI:

12:36 min

October 19, 2010

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Chapters

  • 00:00Title
  • 01:43tRNA Preparation
  • 05:28Expression and Purification of the Recombinant E. histolytica Dnmt2 (Ehmeth) Protein in E. coli BL21
  • 08:06 in vitro tRNA Methylation Assay
  • 10:30Representative Results
  • 12:02Conclusion

Summary

Automatic Translation

이 프로토콜은의 합성 tRNA 기판의 준비에 대해 설명합니다<em> Entamoeba histolytica</em> DNA / tRNA methyltransferase 2 (Dnmt2) 상동체의 Ehmeth과 methyltransferase 활동의 측정합니다. 이 실험 방법은 다른 Dnmt2 단백질의 활동을 조사하고 사용할 수 있습니다.

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