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Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
JoVE Journal
Biology
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JoVE Journal Biology
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
DOI:

06:53 min

January 05, 2011

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Chapters

  • 00:05Title
  • 01:02Lysate Clearance
  • 02:58DNA Binding
  • 04:10DNA Recovery
  • 05:16Quantitative and Qualitative Analyses
  • 06:30Conclusion

Summary

Automatic Translation

This protocol is a cost effective alternative for efficient parallel clarification and plasmid DNA purification from E. coli cultures. The AcroPrep Advance process starts with an optimized lysate clarification filter plate followed by purification on a high binding capacity DNA binding filter plate.

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