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Un lucido e rinforzato Assottigliato cranio-Window a lungo termine Imaging del cervello di topo

Article DOI: 10.3791/3742-v • 09:50 min • March 7th, 2012
March 7th, 2012 •
1, 1, 2,3, 1, 1,4
1Department of Physics, University of California, San Diego, 2Department of Engineering Science and Mechanics, Pennsylvania State University, 3Department of Neurosurgery, Pennsylvania State University, 4Section of Neurobiology, University of California, San Diego

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Presentiamo un metodo per formare una finestra di imaging nel cranio topo che si estende millimetri ed è stabile per mesi senza infiammazione cerebrale. Questo metodo è adatto per studi longitudinali di flusso sanguigno, dinamica cellulare, e cellule / struttura vascolare con microscopia a due fotoni.

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Neuroscience Numero 61 finestra cranica craniotomia la microscopia a due fotoni il flusso di sangue dendrite optogenetics corteccia capillare microglia cronica mouse
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