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En polert og forsterket tynnet-skalle Window for langsiktig Imaging av Mouse Brain
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Chapters
- 00:05Title
- 01:40Surgery Preparation and Animal Mounting
- 04:02The Generation of a Polished and Reinforced Thinned-skull Window
- 07:14Imaging Preparation
- 08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
- 09:30Conclusion
Vi presenterer en metode for å danne en avbildning vindu i musen hodeskallen som spenner millimeter og er stabil i flere måneder uten betennelse i hjernen. Denne metoden egner seg godt for longitudinelle studier av blodstrøm, cellulære dynamikk, og celle / vaskulær struktur ved hjelp av to-foton mikroskopi.
Tags
Polished And Reinforced Thinned-skull WindowLong-term ImagingMouse BrainIn Vivo ImagingCortical FunctionOptical AccessIntracranial EnvironmentPoRTS WindowOptical ClarityBone Regrowth InhibitionLight Scattering ReductionInflammation ReductionTwo-photon Laser Scanning MicroscopyCerebral Blood FlowCellular FunctionAnesthetized PreparationsAwake PreparationsOptogeneticsBlood Flow Disruption
