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Medicine
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プライマリーヒト線維芽細胞の導出のために皮膚パンチ生検植文化
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Medicine
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Please note that all translations are automatically generated.
Click here for the English version.
プライマリーヒト線維芽細胞の導出のために皮膚パンチ生検植文化
DOI:
10.3791/3779-v
•
06:19 min
•
July 07, 2013
•
Malini Vangipuram
,
Dennis Ting
,
Sam Kim
,
Robert Diaz
,
Birgitt Schüle
1
Basic Research Department
,
The Parkinson’s Institute
Chapters
00:01
Title
00:11
Required Materials
01:09
Step 1. Transferring biopsy from tube to cutting dish
02:16
Step 2. Dissection of the skin biopsy
03:28
Step 3. Transfer biopsy pieces to culture plate
05:19
Representative Results
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
ヒト皮膚パンチ生検からの線維芽細胞移植片培養プロトコルは、低継代数で約15〜20万人の細胞バンキングのため4-8週間以内に皮膚細胞を導出するために、技術的に堅牢かつ簡単な方法です。
Tags
Skin Punch Biopsy
Explant Culture
Primary Human Fibroblasts
Disease Study
Induced Pluripotent Stem Cells
Cell-based Functional Assays
Disease Pathways
Drug Screening
Parkinson’s Disease
Enzymatic Procedures
Reproducibility
Technical Simplicity
Time Consideration
Cell Viability
Attachment Of Cells
Dissection
Preparation
Keratinocytes
Article
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