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Skin Punch Biopsi Eksplantering Kultur for Utledning av primære humane fibroblaster
JoVE Journal
Medicine
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JoVE Journal Medicine
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
DOI:

06:19 min

July 07, 2013

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Chapters

  • 00:01Title
  • 00:11Required Materials
  • 01:09Step 1. Transferring biopsy from tube to cutting dish
  • 02:16Step 2. Dissection of the skin biopsy
  • 03:28Step 3. Transfer biopsy pieces to culture plate
  • 05:19Representative Results
  • 06:38Conclusion

Summary

Automatic Translation

Den fibroblast explant kultur protokoll fra human hud slag biopsier er en teknisk robust og enkel måte for å utlede hudceller innen 4-8 uker for banking av ca 15-20 millioner celler ved en lav passasje nummer.

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