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推导人类纤维原细胞的皮肤穿刺活检植文化
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Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Please note that all translations are automatically generated.
Click here for the English version.
推导人类纤维原细胞的皮肤穿刺活检植文化
DOI:
10.3791/3779-v
•
06:19 min
•
July 07, 2013
•
Malini Vangipuram
,
Dennis Ting
,
Sam Kim
,
Robert Diaz
,
Birgitt Schüle
1
Basic Research Department
,
The Parkinson’s Institute
Chapters
00:01
Title
00:11
Required Materials
01:09
Step 1. Transferring biopsy from tube to cutting dish
02:16
Step 2. Dissection of the skin biopsy
03:28
Step 3. Transfer biopsy pieces to culture plate
05:19
Representative Results
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
成纤维细胞植体培养协议,从人体皮肤打孔活检是一种技术上的强大和简单的方法来获取皮肤细胞银行约15-20万个细胞在低通道数的4-8周内。
Tags
Skin Punch Biopsy
Explant Culture
Primary Human Fibroblasts
Disease Study
Induced Pluripotent Stem Cells
Cell-based Functional Assays
Disease Pathways
Drug Screening
Parkinson’s Disease
Enzymatic Procedures
Reproducibility
Technical Simplicity
Time Consideration
Cell Viability
Attachment Of Cells
Dissection
Preparation
Keratinocytes
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