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Journal / Bioengineering / 生きた細胞の細胞内レベルでの秒のタイムスケールの上に小さなGTPase活性の時空間操作…
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Bioengineering

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JoVE Journal Bioengineering
生きた細胞の細胞内レベルでの秒のタイムスケールの上に小さなGTPase活性の時空間操作
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生きた細胞の細胞内レベルでの秒のタイムスケールの上に小さなGTPase活性の時空間操作

Article DOI: 10.3791/3794-v • 10:28 min • March 9th, 2012
March 9th, 2012 •
1, 1, 1,2, 1,2, 2, 1,3, 1
1Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, 2Graduate School of Pharmaceutical Sciences, University of Tokyo, 3Biomedical Engineering, Johns Hopkins University

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光による小さなGTPase活性の時空間制御の方法が記載されている。このメソッドは、ラパマイシン誘発性FKBP-FRBのヘテロと写真ケージシステムに基づいています。光照射の最適化は、細胞内レベルでの低分子量GTPaseの時空間時間的制御の活性化を可能にします。

Tags

バイオ、問題61、低分子量GTPase、ラパマイシン、ケージド化合物、時空間制御、ヘテロ、FKBP、FRB、光照射
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