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Spatio-tidsmæssige Manipulation af Lille GTPase aktivitet på Subcellulær niveau og på Tidsplan for Seconds i levende celler
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Chapters
- 00:05Title
- 02:17Transfection of Plasmid DNA
- 04:02Preparation of cRb-A Solution
- 05:22Microscopy and Light Illumination at the Subcellular Level
- 07:07Examining the Effect of RAC Activation on Local Ruffle Formation
- 09:29Conclusion
En fremgangsmåde til spatio-temporal kontrol lille GTPase-aktivitet af lys, er beskrevet. Denne metode er baseret på rapamycin-induceret FKBP-FRB heterodimerisering og foto-bure systemer. Optimering af lys-bestråling muliggør spatio-tidsmæssigt styret aktivering af små GTPaser på det subcellulære niveau.
Tags
Spatio-temporal ManipulationSmall GTPase ActivitySubcellular LevelSecondsLiving CellsDynamic RegulationRho FamilyGuanosine TriphosphatasesCellular FunctionsVisualizationReal TimePerturbationProtein ActivitiesSubcellular LocationTimingLight-induced ActivationRapamycin-induced FKBP-FRB HeterodimerizationPhoto-caging MethodRapamycinRapid Inducible ActivationInactivationTranslocationPlasma MembraneFRB AnchorPhoto-caging SystemRapamycin Analogs
