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JoVE Journal
Biology

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适用于培养人类胚胎和诱导多能干细胞的小鼠胚胎成纤维细胞的制备
 
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适用于培养人类胚胎和诱导多能干细胞的小鼠胚胎成纤维细胞的制备

Article DOI: 10.3791/3854 09:38 min
June 21st, 2012

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Summary June 21st, 2012

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Click here for the English version.

小鼠胚胎成纤维细胞(MEFs)的质量取决于鼠标CF-1如正确的应变。全能性支持MEFs和条件培养基(厘米),从这些应该包含激活素A,小鬼和TGFβ1的最佳浓度为激活素/ Nodal和FGF通路所需的合作保持自我更新和全能性。

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