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从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

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JoVE Journal Biology

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

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从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

DOI:

10.3791/51406-v

•

09:43 min

•

April 23, 2014

•

In Young Choi, HoTae Lim, Gabsang Lee

¹Institute for Cell Engineering, Department of Neurology and Neuroscience,Johns Hopkins University School of Medicine

Chapters

00:05Title
01:17Transduction
03:40Co-culturing
06:01Expanding the Induced Pluripotent Stem Cells
07:10Results: Induced Pluripotent Stem Cell Characterization
09:12Conclusion

Summary

Automatic Translation

在这里，我们提出我们建立的方法重新编程人类体细胞转基因成无人类iPS细胞与仙台病毒，这说明一致的结果，并提高效率。

Tags

Keywords: Human Induced Pluripotent Stem Cells IPSCs Sendai-virus Integration-free Transgene-free Reprogramming Somatic Cells Genetic Diseases Cell Therapy Drug Screening Disease Modeling High Efficiency Cost-effective

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