Materials
Name | Company | Catalog Number | Comments |
Collagenase I | GIBCO, by Life Technologies | 17100-017 | Use when selecting for T cells |
Collagenase IV | GIBCO, by Life Technologies | 17104-019 | Use for myeloid selection |
DNase | Calbiochem | 260913 | |
RPMI | GIBCO, by Life Technologies | 21870 | |
Dulbecco’s PBS | Lonza Inc. | 17-5158 | |
Fetal Bovine Serum | Lonza Inc. | 14-501F | |
MACs Buffer | 2% FBS for T cells 10% FBS for myeloid cells |
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Thy1.1 PE | eBioscience | 551401 | |
Anti-PE microbeads | Miltenyi Biotec | 120-000-294 | |
Anti-Pan DC microbeads | Miltenyi Biotec | 120-003-183 | |
Anti-CD11b microbeads | Miltenyi Biotec | 120-000-300 | |
LC column | Miltenyi Biotec | 130-042-202 | |
MS column | Miltenyi Biotec | 130-042-201 |
References
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