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En raskere, High Resolution, mtpa-GFP-baserte Mitokondrie Fusion Assay Innhente Kinetic Data om flere celler i parallell Bruke konfokalmikroskopi
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En raskere, High Resolution, mtpa-GFP-baserte Mitokondrie Fusion Assay Innhente Kinetic Data om flere celler i parallell Bruke konfokalmikroskopi

Article DOI: 10.3791/3991-v • 10:45 min • July 20th, 2012
July 20th, 2012 •
1, 2, 3, 3, 3
1Department of Neuroscience, Center for Neuroscience Research, Tufts School of Medicine, 2Department of Internal Medicine, Geriatrics & Gerontology, Wake Forest Baptist Medical Center, 3Department of Medicine, Boston University Medical Center

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Mitokondrie fusjon ble målt ved å spore ekvilibrering av photoconverted matrix-målrettet GFP over mitokondrie nettverket over tid. Så langt, kunne bare en celle må utsettes for en times lang kinetisk analyse av gangen. Vi presenterer en metode som samtidig måler flere celler, og dermed påskynde datainnsamlingen.

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Molecular Biology genetikk cellebiologi fysikk konfokalmikroskopi mitokondriene fusion TMRE mtPAGFP INS1 mitokondrielle dynamikk mitokondrie morfologi mitokondriell nettverk
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